Direct in Vitro Shoot Regeneration of Cicer Arietinum
L. from Decapitated Embryo Axes Explants
Journal:
GRENZE International Journal of Engineering and Technology
Authors:
Jaya Srivastava, Pallavi Sinha
Volume:
3
Issue:
1
Grenze ID:
01.GIJET.3.1.39
Pages:
36-39
Abstract
This study reports a protocol for plant regeneration from cultured explants of
chickpea Cicer arietinum L. via direct organogenesis using decapitated embryonic axes
explants. Morphologically normal healthy plants were regenerated directly without an
intervening callus phase from the explants. Explants prepared from overnight soaked seeds
were cultured on Murashige and Skoog (MS) medium fortified with different concentrations
of benzylaminopurine (BAP), indolbutyric acid (IBA) and naphthalene acetic acid (NAA).
The type and concentration of plant growth regulators influenced the frequency of multiple
shoot regeneration. 2 mg/L BAP and 0.05mg/L IBA was the most effective combination for
high frequency multiple shoot induction. Individual shoots produced were aseptically
excised and sub cultured in the media fortified with 1 mg/L GA3 for shoot elongation. The
elongated shoots were grafted on the rootstocks prepared from the seeds of the same
cultivar.