Abstract

One of the most common and prominent stains in histology is Hematoxylin and eosin stain also spelled as haematoxylin and eosin stain and abbreviated as H and E stain or HE stain. It is used as the gold standard in staining the biopsy tissue for detection of presence of malignant cells. Use of H and E stain has been more than a century in histological cancer detection. In this paper we give the introduction on H and E stain. We also discuss the procedure of applying this stain and provide the alternative to H and E dye.